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KMID : 0545120180280020298
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 2 p.298 ~ p.304
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Bioconversion of Tetracycline Antibiotics to Novel Glucoside Derivatives by Single-Vessel Multienzymatic Glycosylation
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Pandey Ramesh Prasad
Chu Luan Luong
Kim Tae-Su
Sohng Jae-Kyung
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Abstract
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The single-vessel multienzyme UDP-α-D-glucose recycling system was coupled with a forward glucosylation reaction to produce novel glucose moiety-conjugated derivatives of different tetracycline antibiotic analogs. Among five tetracycline analogs used for the reaction, four molecules (chlorotetracycline, doxytetracycline, meclotetracycline, and minotetracycline) were accepted by a glycosyltransferase enzyme, YjiC, from Bacillus licheniformis to produce glucoside derivatives. However, the enzyme was unable to conjugate sugar units to rolitetracycline. All glucosides of tetracycline derivatives were characterized by ultraviolet absorbance maxima, ultra-pressure liquid chromatography coupled with photodiode array, and high-resolution quadruple time-of-flight electrospray mass spectrometry analyses. These synthesized glucosides are novel tetracycline derivatives.
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KEYWORD
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Novel tetracycline derivatives, glycosylation, single-vessel multienzyme reaction, UDP-α-D-glucose recycling system
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